Summary
The neuraminidase-inhibition (NI) assay is a laboratory procedure for the identification of the neuraminidase (NA) glycoprotein subtype in influenza viruses or the NA subtype specificity of antibodies to influenza virus. A serological procedure for subtyping the NA glycoprotein is critical for the identification and classification of avian influenza (AI) viruses. The macro-procedure was first described in 1961 by D. Aminoff et al. [2] and was later modified to a microtiter plate procedure (micro-NI) by Van Deusen et al. [4]. The micro-NI procedure reduces the quantity of reagents required, permits the antigenic classification of many isolates simultaneously, and eliminates the spectrophotometric interpretation of results. Although the macro-NI has been shown to be more sensitive than the micro-NI, the micro-NI test is very suitable for testing sera for the presence of NA antibodies and has proven to be a practical and rapid method for virus classification. This chapter will provide an overview of the USDA-validated micro-NI procedure for the identification of subtype-specific NA in AIV and antibodies.
This is a preview of subscription content, log in via an institution to check access.
Access this chapter
Tax calculation will be finalised at checkout
Purchases are for personal use only
References
1. Aymard-Henry, M., Coleman, M. T., Dowdle, W. R., Laver, W. G., Schild, G. C., and Webster, R. G. (1973) Influenza virus neuraminidase and neuraminidase-inhibition test procedures. Bull. WHO 48, 199–202.
2. Aminoff, D. (1961) Methods for the quantitative estimation of N-acetyl neuraminic acid and their application to hydrolysates of sialomucoids. Biochem. J. 81, 384.
3. Palmer, D. F., Coleman, M. T., Dowdle, W. R., and Schild, G. C. (eds.) (1975) Advanced Laboratory Techniques for Influenza Diagnosis. Procedural Guide, Immunology Series No. 6. Atlanta: U.S. Dept. of Health, Education, and Welfare.
4. Van Deusen, R. A., Hinshaw, V. S., Senne, D. S., and Pellacani, D. (1983) Micro neuraminidase-inhibitions assay for classification of influenza A virus neuraminidase. Avian Dis. 27(3), 745–750.
5. Swayne, D. E. and Halvorson, D. A. (2003) Influenza, in Diseases of Poultry, 11th ed. (Y. M. Saif, H. J. Barnes, J. R. Gilsson, A. M. Fadly, L. R. McDougald, and D. E. Swayne, eds.). Ames: Iowa State University Press, pp. 135–155.
6. Swayne, D. E., Senne, D. A., and Beard, C. W. (1998) Avian influenza, in A Laboratory Manual for the Isolation and Identification of Avian Pathogens, 4th ed. (D. E. Swayne, J. R. Glisson, M. W. Jackwood, J. E. Pearson, and W. M. Reed, eds.). Kennett Square, PA: American Association of Avian Pathologists, pp. 150–155.
7. Webster, R. G. and Campbell, C. H. (1972) An inhibition test for identifying the neuraminidase antigen on influenza viruses. Avian Dis. 16, 1057–1066.
Author information
Authors and Affiliations
Editor information
Editors and Affiliations
Rights and permissions
Copyright information
© 2008 Humana Press, a part of Springer Science+Business Media, LLC
About this protocol
Cite this protocol
Pedersen, J.C. (2008). Neuraminidase-Inhibition Assay for the Identification of Influenza A Virus Neuraminidase Subtype or Neuraminidase Antibody Specificity. In: Spackman, E. (eds) Avian Influenza Virus. Methods in Molecular Biology™, vol 436. Humana Press. https://doi.org/10.1007/978-1-59745-279-3_9
Download citation
DOI: https://doi.org/10.1007/978-1-59745-279-3_9
Publisher Name: Humana Press
Print ISBN: 978-1-58829-939-0
Online ISBN: 978-1-59745-279-3
eBook Packages: Springer Protocols