Barilius barila, is a vulnerable and high valued edible Eastern Himalayan hill stream fish of Terai and Dooars regions of northern region of West Bengal. The study presents an experimental work on the captive breeding through induced breeding using synthetic hormone in Barilius barila carried for over a period of one year. This fish spawned in captivity only in running water system with a dosage of 0.5ml/fish WOVA-FH, a synthetic hormone. The results showed fecundity to range from 1440 to 7050 with an average rate of 5020.The average Gonado-somatic Index of Barilius barila for female was 12.77 and for male 6.69. Gonado-Somatic Index was higher in female than male. Gastro-somatic Index ranged from 5.94 to 11.73. The present work contributed to the lacunae in the information on fecundity, Gonadosomatic Index and spawning biology of Barilius barila.

The present study was aimed to perform induced breeding of Telescopic eye gold fish (Carassius auratus auratus) in captivity by administering WOVA-FH hormone at different doses. The study was conducted during the period from January-March, 2018 at Chennai, Tamil Nadu for 3 months. All the breeding pairs were bred including control. Significant (<0.05) difference were observed in the success rate of spawning at different dose among treatments and in control. WOVAFH at a dose of 0.7ml/kg and 1.4ml/Kg was found to be effective for male and female respectively with 74% fertilization rate, 82 % hatching rate and fecundity of 1185-1205 eggs/female. Variability in hormone dose induced resulted in variability in latency period (5-9hrs), fecundity (767-1205 eggs/female), fertilization rate (46-74%) and hatching rate (52-82%). The positive response to synthetic hormone (WOVA-FH) with considerable fecundity, fertilization and hatching rate makes it possible to conduct the breeding program o...

Induced Breeding (IB) is the most significant advancements in the field of aquaculture to induce reproduction in fish. It is a ttechnique to stimulate ripe fish breeders by pituitary hormone or any other synthetic hormone to breed in captive condition by promotion of timely release of sperms and eggs. Identification of donor fish can be done on the basis of Secondary Sexual characters which are prominent in the breeding season of fishes. The preparation of carp pituitary extract the glands are removed carefully from freshly killed the fish called donor fish. For best result the donor fish should be fully ripe and mature. Common carp is the best donor fish, because it breeds throughout the year and the individuals are available in all parts of the world.

Bangana devdevi (Hora) is an indigenous medium-sized and bentho-pelagic minor carp, widely distributed in the Chindwin headwaters of Manipur, Northeast India. Induced breeding of B. devdevi was done using Gonopro-FH in different doses @ 0.4ml, 0.5ml and 0.6ml/kg body weight in a set of three experimental groups T1, T2 and T3, respectively. All the males were given half the doses of females. Spawning commenced 6–10 hr after injection and was completed within 4–5 hr. Fertilized eggs were hatched out after 11–16 hr of fertilization at temperatures of 26.4–27.5°C. Statistical analysis was carried out to determine the relation between the hormone dosage with egg output, fertilisation rate and hatching rate. The highest number of fertilisation (94.25%) and hatching rate (89.03%) were found in fish with Gonopro-FH @ 0.5ml/kg body weight female and significantly higher (P<0.05) than T1 and T3. The present study may be beneficial for species conservation and management strategies in river...

An experiment on induced breeding of Bheda/Meni fish (Nandus nandus) was carried out to determine the optimum dose of pituitary gland hormone during December 2018 to June 2019 at Floodplain Sub-station of Bangladesh Fisheries Research Institute (BFRI), Santahar, Bogura. In this experiment, PG was used as inducing agent for both male and female fish. For induced breeding, PG was administered at the rate of 1, 2 and 3 mg/kg body weight to male brood fish, and correspondingly 2, 4 and 6 mg/kg body weight to female brood fish that were considered as the treatments namely, T1, T2 and T3, respectively. Fifty pairs of male and female fish were collected from the Raktadaha beel from where forty-five pairs were selected for induced breeding and the average body weight of the male and female were 43.0±2.5 gm and 55.2±3.0 gm in T1, 45.1±2.9 gm and 56.0±2.8 gm in T2, and 40.3±2.4 gm and 52.6±3.2 gm in T3. The male-female ratio was maintained as 1:1 in the breeding hapa. It was observed that fem...

The striped dwarf catfish, Mystus vittatus, a small indigenous fish of Bangladesh is scarce facing anthropogenic interventions. The present study depicts the captive breeding of M. vittatus applying different stimulating hormones which might improve the production. For this purpose, 400 wild brood fish of M. vittatus were collected and some breeding parameters such as gonado-somatic index (GSI), hepato-somatic index (HSI) and fecundity were measured at 15 days interval at each month during a year. For captive breeding, the broods were kept in the containers dividing into two different groups (male: female sex ratio 1:1 and 1:2) treated with carp pituitary gland (CPG: 4 to 12 mg/kg), flash (S-GnRHa: 0.4 to 1.2 mL/kg) and CPG plus flash hormones (2+0.2 to 6+0.6 mg-mL/kg) by 15 different doses and planned with a single dose for male and female. A control unit with no hormone was designed for each sex ratio. For evaluating the breeding performance, fertilization rate, hatching rate and survival rate of larvae were compared. In addition, the water quality parameters (temperature, DO, TDS, and pH) of incubators were checked. Maximum GSI value (25.54±5.86%) was found in mid-July and minimum (0.11±0.01%) was in mid-October, whereas the HSI value was lowest in mid-July for both female (1.61±0.11%) and male (2.40±0.08%). The average fecundity was 16175±10803 from end-March to end-September whereas the highest and lowest values were 32794±1284 in mid-July and 2109±412 in mid-September. Based on the GSI and HSI values of male and female, mid-July is the spawning season of this species. The higher latency period (8-9 hrs.) was noted with CPG and lower (6-7 hrs.) with the CPG plus flash hormone. The highest fertilization rate (92.6±6.38%), hatching rate (78.4±5.73%) and survival rate (69±7.03%) were found with a dose of 3+0.3 mg+mL/kg (CD2) of fish at the sex ratio of 1:2 whereas the average water parameter of temperature, DO, pH and TDS were 29.72±0.30 º C, 8.61±0.08 mg/L, 7.46±0.01, 180.49±18.53 mg/L. In captive conditions, the seed of M. vittatus can be mass-produced and helpful to aquaculture and conservation through this research.

Induced breeding trial of two years old striped mullet (Mugil cephalus L.) was conducted during winter season in a commercial fish hatchery at Cox’s Bazar, Bangladesh. Adult or sub-adults of M. cephalus were collected from the wild and reared in the saline ponds from August to December 2014. The broods were injected using carp PG, HCG and LRH-A2 after 48 hrs of acclimatization in the hatchery tanks. The effective doses of carp PG 30mg/kg body weight, LRH-A2150?g with a combination of 0.3ml Domperidone and 0.5 ml Calcium injection, and HCG dose of 30,000 IU in case of female and 5,000 IU in case of male were found to spawning success. The GSI value ranged between 7.92-12.38 and egg diameter of matured fish ranged between 563-594?m and that of fertilized egg between 650-680?m with fecundity calculated as 780 to 900 nos/g. The fish started spawning between 44-48 hrs and cell division was observed after the first hour of spawning, however, mass mortality occurred after 6 hrs of spawning...

An experiment on induced breeding of gang magur, Hemibagrus menoda (Hamilton, 1822) using Ovatide hormone was carried out at in the Field Laboratory Complex of Faculty of Fisheries, Bangladesh Agricultural University, Mymensingh. This study consists of three treatments each with three replications. The objective of the experiment was to find out the effective dose of Ovatide hormone for induced breeding. A total number of 54 brood fish were used for the experiment among which 36 were male and 18 were female. Brood fish were kept in the ratio of 2♂:1♀ for breeding purpose. Female brood fish were injected at the rate of 7, 5, 3 ml Ovatide/kg body weight while the males were injected with 3, 2.5 and 1.5 ml Ovatide/kg body weight respectively in T1, T2, and T3 at the same time. The brood fish were injected with single dose of Ovatide in all treatments. Ovulation rates were 0%, 100%, 63%; fertilization rates were 0%, 97%, and 90%, and hatching rates were 0%, 95% and 76% in treatments T1,...

Present investigation was conducted to investigate the induced spawning practices in 21 hatcheries of 7 Upazilas of Barisal District in Bangladesh. It was observed that, 5 native fish species viz. rui (Labeo rohita), catla (Catla catla), mrigal (Cirrhina mrigala), bata (Labeo bata), sarpunti (Puntius sarana) and 5 exotic fish species namely silver carp (Hypophthalmichthys moltrix), bighead carp (Aristichthys nobilis), grass carp (Ctenopharyngodon idella), common carp (Cyprinus carpio) and mirror carp (Cyprinus carpio var. specularis) were used in the surveyed hatcheries for induced spawning purpose. The weight of different brood fish was ranged from 0.25 kg to more than 8kg and age was ranged from 1 to 10 years for different fishes. Two types of hormone injections like PG (pituitary gland) and HCG (human chorionic gonadotropin) were used for induced spawning. The rate of 1 st doses of injections of PG were from 1 to 4 mg/kg and HCG from 150 to 500 IU/kg and the rate of 2 nd doses of injections of PG for native and exotic species were from 4 to 8 mg/kg and 4 to 10 mg/kg, respectively. The ovulation period and hatching rate in different fish species varied from 1.5 to 6 hours and 47% to 86%, respectively.

The book describes the reproductive endocrinology of fish, followed by the induction of spawning by injection of gonadotropins and its practical application to several species used in aquaculture. Assessment of sexual maturity in broodstock, spontaneous spawning in ...